Cell Culture:Article Title: Survivin modulates stiffness-induced vascular smooth muscle cell motility
Article Snippet: .. Human vascular smooth muscle cells (VSMCs; catalog number [Cat. No.] 354-05a, Cell Applications, Inc.) were cultured in Dulbecco's modified Eagle's medium (DMEM; Cat. No. 10-014-CV, Corning) supplemented with 10% fetal bovine serum (FBS; Cat. No. 2510268RP, GIBCO), 1 mM sodium pyruvate (Cat. No. S8636, Sigma-Aldrich), 1% penicillin–streptomycin (Cat. No. 30-002-CI, Corning), 50 μg/ml gentamicin (Cat. No. 30-005-CR, Corning), and 2% minimum essential media (MEM) amino acids (Cat. No. M5550, Sigma-Aldrich). ..
Article Title: Engineering a Matrix-Preserving Vascular dECM Platform with Tunable Stiffness for In Vitro Vascular Remodeling
Article Snippet: For histologic comparison of tissue structure before and after decellularization, native and decellularized porcine aorta samples were stained with hematoxylin and eosin (H&E; hematoxylin solution, Sigma-Aldrich, GHS232; eosin Y solution, Sigma-Aldrich, HT110132) and PSR staining. .. Human vascular smooth muscle cells (VSMCs; Cell Applications, Inc., 354-05a) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, 11885084) supplemented with 10% fetal bovine serum (FBS; GIBCO, 2510268RP), 1 mM sodium pyruvate (Sigma-Aldrich, S8636), 1% penicillin-streptomycin (Corning, 30-002-CI), 50 μg/ml gentamicin (Corning, 30-005- CR) and 2% MEM amino acids (Sigma-Aldrich, M5550) at 37°C and 10% CO 2 . ..
Article Title: Survivin modulates stiffness-induced vascular smooth muscle cell motility
Article Snippet: .. Human vascular smooth muscle cells (VSMCs; catalog number [cat. no.] 354-05a, Cell Applications, Inc.) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; cat. no. 10-014-CV, Corning) supplemented with 10% fetal bovine serum (FBS; cat. no. 2510268RP, GIBCO), 1 mM sodium pyruvate (cat. no. S8636, Sigma), 1% penicillin-streptomycin (cat. no. 30-002-CI, Corning), 50 μg/ml gentamicin (cat. no. 30-005-CR, Corning) and 2% MEM amino acids (cat. no. M5550, Sigma). ..
Modification:Article Title: Survivin modulates stiffness-induced vascular smooth muscle cell motility
Article Snippet: .. Human vascular smooth muscle cells (VSMCs; catalog number [Cat. No.] 354-05a, Cell Applications, Inc.) were cultured in Dulbecco's modified Eagle's medium (DMEM; Cat. No. 10-014-CV, Corning) supplemented with 10% fetal bovine serum (FBS; Cat. No. 2510268RP, GIBCO), 1 mM sodium pyruvate (Cat. No. S8636, Sigma-Aldrich), 1% penicillin–streptomycin (Cat. No. 30-002-CI, Corning), 50 μg/ml gentamicin (Cat. No. 30-005-CR, Corning), and 2% minimum essential media (MEM) amino acids (Cat. No. M5550, Sigma-Aldrich). ..
Article Title: Engineering a Matrix-Preserving Vascular dECM Platform with Tunable Stiffness for In Vitro Vascular Remodeling
Article Snippet: For histologic comparison of tissue structure before and after decellularization, native and decellularized porcine aorta samples were stained with hematoxylin and eosin (H&E; hematoxylin solution, Sigma-Aldrich, GHS232; eosin Y solution, Sigma-Aldrich, HT110132) and PSR staining. .. Human vascular smooth muscle cells (VSMCs; Cell Applications, Inc., 354-05a) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; GIBCO, 11885084) supplemented with 10% fetal bovine serum (FBS; GIBCO, 2510268RP), 1 mM sodium pyruvate (Sigma-Aldrich, S8636), 1% penicillin-streptomycin (Corning, 30-002-CI), 50 μg/ml gentamicin (Corning, 30-005- CR) and 2% MEM amino acids (Sigma-Aldrich, M5550) at 37°C and 10% CO 2 . ..
Article Title: Survivin modulates stiffness-induced vascular smooth muscle cell motility
Article Snippet: .. Human vascular smooth muscle cells (VSMCs; catalog number [cat. no.] 354-05a, Cell Applications, Inc.) were cultured in Dulbecco’s modified Eagle’s medium (DMEM; cat. no. 10-014-CV, Corning) supplemented with 10% fetal bovine serum (FBS; cat. no. 2510268RP, GIBCO), 1 mM sodium pyruvate (cat. no. S8636, Sigma), 1% penicillin-streptomycin (cat. no. 30-002-CI, Corning), 50 μg/ml gentamicin (cat. no. 30-005-CR, Corning) and 2% MEM amino acids (cat. no. M5550, Sigma). ..
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